Hygromycin resistance as a selectable marker in Dictyostelium discoideum.

Self-induced nystatin resistance in Dictyostelium discoideum.

The cellular slime mold Dictyostelium discoideum is normally susceptible to the action of the polyene antibiotic nystatin. We describe conditions under which transient nystatin resistance can be induced in the absence of detectable changes in sterol composition. These results suggest that nystatin plays a positive role in promoting resistance to itself.

Dictyostelium discoideum

Dictyostelium discoideum is a cellular slime mold [4] that serves as an important model organism [5] in a variety of fields. Cellular slime molds have an unusual life cycle. They exist as separate amoebae, but after consuming all the bacteria in their area they proceed to stream together to form a multicellular organism. These features make them a valuable tool for studying developmental proces...

influx in Dictyostelium discoideum

Translocations in Dictyostelium discoideum.

Fourteen translocations of independent origin were identified in Dictyostelium discoideum on the basis of segregation anomalies of diploids heterozygous for these chromosome rearrangements, all of which led to the cosegregation of unlinked markers. Many of these translocations were discovered in strains mutagenized with MNNG or in strains carrying mutations affecting DNA repair; however, sponta...

Dictyostelium Discoideum: A Developmental System

cernible. The peroxidase technique also permits the specimen to be examined by electron microscopy. Perhaps a few too many chapters are devoted to individual applications of the technique, but it is an exciting development in diagnostic virology. Viral genomes can be present in cells without full expression leading to the production of viral antigens or virions which can be detected by immunolo...